Abstract. Amino acid composition of 5 local and imported sesame seed cultivars (Sesamun indicum L.) grown in Vietnam (yellow Quang Tri sesame,
yellow Thua Thien Hue sesame, white Japanese sesame, black Thua Thien
Hue sesame, black Thanh Hoa - Quang Tri sesame) were analyzed by derivatization with ortho-phthaladehyt (OPA) for primary amino acid and 9-
fluorenyl methyl chloroformat (FMOC) for secondary amino acids on HPAmino Quant series II (Hewlett Packard). The results showed that the total
amino acid contents in the seeds of these cultivars were approximately 17.25
- 21.98%. The total amino acid cotents of the Japanese sesame cultivars
were higher than those of the local Vietnamese ones. Among the local Vietnamese sesame cultivars, the total amino acid contents of the black Thanh
Hoa - Quang Tri sesame were the highest and approximately similar to the
Japanese ones. The sesame seed proteins were characterized by high nutritional value with high ratios of essential amino acids in comparison with
the FAO standard protein. The sesame seed proteins were rich in essential
amino acids, especially lysine. No significant differences were found between
2 analyzed sesame types: yellow and black.
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JOURNAL OF SCIENCE OF HNUE
Natural Sci., 2008, Vol. 53, N
◦
. 5, pp. 122-127
COMPARISON OF AMINO ACID COMPOSITION, NUTRITIONAL
VALUE OF SESAME SEED PROTEINS IN SOME LOCAL
AND IMPORTED SESAME CULTIVARS IN VIETNAM
Tran Thi Thanh Huyen, Nguyen Nhu Khanh
Nguyen Thi Lan Phuong and Hoang Thi Thu Phuong
Hanoi National University of Education
Abstract. Amino acid composition of 5 local and imported sesame seed cul-
tivars (Sesamun indicum L.) grown in Vietnam (yellow Quang Tri sesame,
yellow Thua Thien Hue sesame, white Japanese sesame, black Thua Thien
Hue sesame, black Thanh Hoa - Quang Tri sesame) were analyzed by deriva-
tization with ortho-phthaladehyt (OPA) for primary amino acid and 9-
fluorenyl methyl chloroformat (FMOC) for secondary amino acids on HP-
Amino Quant series II (Hewlett Packard). The results showed that the total
amino acid contents in the seeds of these cultivars were approximately 17.25
- 21.98%. The total amino acid cotents of the Japanese sesame cultivars
were higher than those of the local Vietnamese ones. Among the local Viet-
namese sesame cultivars, the total amino acid contents of the black Thanh
Hoa - Quang Tri sesame were the highest and approximately similar to the
Japanese ones. The sesame seed proteins were characterized by high nutri-
tional value with high ratios of essential amino acids in comparison with
the FAO standard protein. The sesame seed proteins were rich in essential
amino acids, especially lysine. No significant differences were found between
2 analyzed sesame types: yellow and black.
1. Introduction
Sesame (Sesamum indicum L.) is grown for short term oil producing seeds
and is of potential development for its nutritional values. Sesame is known as the
queen of oil producing cultivar because the oil ratio of sesame seeds takes up 44 -
52% [5]. Sesame oil contains much vitamin E, vitamin B [3], unsaturated fatty acids
(oleic and linoleic), which are able to control levels of blood cholesterol [5]. Protein
in sesame seeds, approximately 26.25% [3], with high nutritional values, is used as a
valuable source of plant proteins. Especially, presence of most essential amino acids
122
Comparison of amino acid composition, nutritional value of sesame seed proteins...
in protein of sesame seeds has contributed to increased nutritional values of sesame
seeds. The comparision of amino acids in sesame seeds and in meat indicated that
amino acids in sesame seeds are nearly as high as those in meat [3].
Protein in sesame seeds is also essential to human health [1]. The use of α-
globulin, isolated from sesame protein, with low levels of lysine/arginine, can sub-
stantially reduce cholesterol and other harmful fats present in the blood [6]. In this
experiment, we selected some local sesame cultivars, the one imported from Japan
for analysis and evaluation of nutritional quality of sesame seeds. The analysis of
amino acid composition in sesame seeds and ratios among essential amino acids in
protein of sesame seeds is the basis to select high quality sesame cultivars that are
farmed and used in our country.
2. Content
2.1. Sources and methodology
2.1.1. Sources
Sesame cultivars were provided by the Department of Seed Gene Bank Viet-
nam Institute of Agriculture Science and Technology. These cultivars, which have
been tested, selected and publicized, including: yellow Quang Tri sesame, yellow
Thua Thien Hue sesame, white Japanese sesame, black Thua Thien Hue sesame,
black Thanh Hoa - Quang Tri sesame
Sesame seeds that were cleaned, dried, and kept at 4
0
C, which were used for
the analysis.
2.1.2. Determination of amino acid content
Amino acid composition of sesame seed was analyzed by derivatization with
Ortho-phthaladehyt (OPA) for primary amino acids and 9-fluorenyl methyl chloro-
format (FMOC) for secondary amino acids on HP-Amino Quant Series II (Hewlett
Packard). Samples were processed by hydrolysis.
2.2. Results and discussions
2.2.1. Composition and content of amino acids in sesame seeds of stud-
ied cultivars
The results of amino acid composition of sesame seed analysed after the hydrol-
ysis with 6N HCl solution supplemented by phenol at 110
0
C in 24 hours indicated
that sesame seed proteins contain all amino (except tryptophan that was identified
due to complete splitting during the hydrolysis. See Figure 1, 2 and 3).
123
Tran Thi Thanh Huyen, Nguyen Nhu Khanh, Nguyen Thi Lan Phuong
and Hoang Thi Thu Phuong
Figure 1. Amino acid composition
of yellow Thua Thien Hue sesame
Figure 2. Amino acid composition
of white Japanese sesame
Figure 3. Amino acid composition
of black Thanh Hoa - Quang Tri sesame
124
Comparison of amino acid composition, nutritional value of sesame seed proteins...
Table 1. Composition and content of amino acids in sesame seed cultivars
(g of amino acid per 100 g of seed)
No. Amino acid
Yellow
QT
Yellow
TTH
White
NB
Black
TTH
Black
TH-QT
1 Aspartic acid 1.64 1.35 1.70 1.73 1.51
2
Glutamic acid 4.03 3.17 4.88 4.97 4.48
3
Serine 0.99 0.81 1.02 1.00 1.13
4
Histidine 0.68 0.61 0.07 0.68 0.64
5
Glycine 1.19 1.00 1.25 1.24 1.22
6 Threonine 0.82 0.71 0.85 0.83 0.83
7 Alanine 1.03 0.86 1.07 1.06 1.04
8 Arginine 2.69 2.17 2.80 1.93 2.53
9 Tyrosine 0.52 0.44 0.54 0.63 0.53
10 Cysteine + Xystin 0.25 0.23 0.26 0.26 0.26
11 Valine 1.14 0.99 1.18 1.13 1.18
12
Methionine 0.44 0.49 0.50 0.44 0.67
13
Phenylalanine 1.11 0.93 1.14 1.13 1.16
14
Isoleucine 0.80 0.69 0.85 0.82 0.86
15
Leucine 1.53 1.32 1.59 1.56 1.63
16 Lysine 0.95 0.85 0.99 0.96 1.23
17 Proline 0.71 0.63 0.66 0.75 0.70
Total 20.52 17.25 21.98 21.12 21.60
The results of amino acid content of sesame seed in the Table 1 are shown as
follows:
Amino acid content in total in the sesame cultivars analysed ranges 17.25
21.98%. The white Japanese sesame is the highest in amino acid content of 21.98%.
Meanwhile other local sesame cultivars are lower in the total amino acid content.
The levels of yellow Thua Thien Hue sesame, black Thua Thien Hue sesame, yellow
Quang Tri sesame, black Thanh Hoa - Quang Tri sesame are 17.25%, 21.12%, 20.52%,
21.60% respectively.
Of the local sesame cultivars analysed, black Thanh Hoa - Quang Tri sesame
has the highest amino acid content, which is almost equivalent to that of the im-
ported white Japanese sesame.
No significant differences in the total amino acid content between the white
sesame and the black sesame is found.
The total amino acid content in sesame seed is three times as high as it is in
rice grain (approximately 7 - 8%), half of that in the soya seed (approximately 35 -
40%) and a little lower than that in mung bean seed (22 - 25%) [4].
125
Tran Thi Thanh Huyen, Nguyen Nhu Khanh, Nguyen Thi Lan Phuong
and Hoang Thi Thu Phuong
As in other plant proteins, sesame protein is rich in amino acids: Glutamic
acid, Aspartic acid, Arginine, etc. Lysine content in the sesame seed is as high as
0.85 - 1.25%. These factors are characterized by its preeminence [2].
No difference in the total amino acid content between the black sesame and
the yellow sesame is found.
2.2.2. Nutritional values of proteins in studied sesame seeds
The sesame seed proteins were characterized by high nutritional value with
the high ratios of essential amino acids present in that protein.
Amino acid content of proteins in sesame seeds is shown in Table 2.
Table 2. Amino acid content of proteins in sesame seeds
(g of amino acid per 100 g of protein)
STT Amino acid
Yellow
QT
Yellow
TTH
White
NB
Black
TTH
Black
TH-QT
FAO
Standard
1 Aspartic acid 7.99 7.83 7.73 8.19 6.99
2 Glutamic acid 19.64 18.38 22.20 23.53 20.74
3
Serine 4.82 4.69 4.64 4.73 5.23
4
Histidine 3.31 3.54 3.18 3.22 2.96
5
Glycine 5.79 5.79 5.69 5.87 5.65
6
Threonine 3.99 4.12 3.87 3.93 3.84 2.80
7 Alanine 5.02 4.98 4.67 5.02 4.81
8 Arginine 13.11 12.58 12.74 9.14 11.76
9 Tyrosine 2.53 2.55 2.47 2.98 2.45
10 Cysteine + Xystin 1.22 1.33 1.18 1.23 1.20
11 Valine 5.56 5.74 5.37 5.35 5.46 4.20
12 Methionine 2.14 2.84 2.27 2.08 3.10 2.20
13
Phenylalanine 5.41 5.39 5.19 5.35 5.37 2.80
14
Isoleucine 3.89 4.00 3.87 3.88 3.98 4.20
15
Leucine 7.46 7.65 7.23 7.39 7.55 4.20
16
Lysine 4.63 4.93 4.50 4.55 5.69 4.20
17 Proline 3.46 3.65 3.00 3.55 3.24
Data in the Table 2 indicates that ratios of essential amino acids in proteins of
analysed sesame seeds are similar (methionine, lysine) or higher (threonine, valine,
phenylalanine, leucine) than in the FAO standard proteins [7]. No clear difference
in protein quality of the black and yellow sesame cultivars is found.
126
Comparison of amino acid composition, nutritional value of sesame seed proteins...
3. Conclusion
1- The total amino acid content in the sesame cultivars studied ranges 17.25
- 21.98%. The imported Japanese cultivar has the highest content.
2- The black Thanh Hoa - Quang Tri sesame, one of the four local cultivars
studied, is the highest in the total amino acid content almost as high as it is in
white Japan sesame.
3- Protein in the black sesame is high in nutritional value and in essential
amino acids.
4- There is no clear difference in quality and content of amino acids in the
yellow and black sesame cultivars.
REFERENCES
[1] Dang Thi Ly, Tu Giay, Tu Ngu, Le Thi Thai, 1997. Use of sesame powder
to prepare food for babies at 12-36 months of age. Journal of Preventive Medicine,
Vol. 7, N
◦
. 4, pp. 78-80, (in Vietnamese).
[2] Nguyen Thi Ty, Tong Quynh Mai, Nguyen Bich Nhi, Phan Van Chi, 2003.
Amino acid composition and nutritional values of protein in local and imported
sesame cultivars in Vietnam. Biology Magazine, N
◦
. 9, pp. 71-76, (in Vietnamese).
[3] Le Quang Vuong, Hoang Van Son, Phan Xuan Thieu, 2005. Bio-chemical
and food indexes of three sesame cultivars in the coastal sand area in Nghe An
province. Vinh University, (in Vietnamese).
[4] Nguyen Vy, 2003. Sesame. Nghe An Publishing House, (in Vietnamese).
[5] Vijayarajan Sharmila, Siruthaiyur Krishnamoorthy Ganesh, Mahalingam
Gunasekaran, 2007.Generation mean analysis for quantitative traits in sesame (Sesa-
mum indicum L.) crosses. Genetics and Molecular Biology, Vol. 30, N
◦
. 1, pp. 80-84.
[6] Praksh,V and Nandi, P. K., 1982. Studies on the conformation of α- globulin
from Sesamum indicum L. in cationic and nonionic detergents. J. Biosci, Vol. 4, N
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3, pp. 331-345, India.
[7] FAO, 1976. Hand Book on human requirements in food suffs FAO. Geneve.
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